The main purpose of the study was to investigate the mental toughness and depression between male and female players. The data pertaining to the study were collected by two standard questionnaires, Mental Toughness designed by Dr. Alan Goldberg and depression inventory framed by Prof. O. P. Mishra, Dr. Vidya B. Verma, & Santosh Kumar was used. For the present study, the data were collected from the inter-collegiate of male and female players of different districts of Kashmir division. The data pertaining to mental toughness and depression collected from 120 subjects and 60 subjects were selected from each group i.e. 60 from male and 60 female players, through purposive random sampling for testing the hypothesis. The data obtained from the responses given by the subjects on standard questionnaire of mental toughness and Depression was marked according to the key and analyzing by using‘t’ test to find out the significant difference in Mental toughness and Depression between male and female players at 0.5 level of significance. It was hypothesized that there would significant difference in mental toughness and depression between male and female players.