Environmental contamination due to persistence of pesticide residues is gaining worldwide attention. Exposure to pesticide residues can represent a potential risk to any organisms. The residue level of pesticides were quantified in 2 lakes during the year 2017, water samples from the Byramangala and Mulbagal lake region of Southern India. Samples were extracted for pesticide preparation prior to analysis with Gas Chromatography. A total of 17 Organochlorine pesticides were examined. The experimental results revealed that, isomers of Alpha HCH & 4,4-DDD and Endrin Aldelyde of Byramangala lake were detected and doesn’t find pesticide residues from Mulbagal lake. Detection of pesticide residues shows serious threat to the ecosystem. Thus, water bodies in the vicinity of the Byramangala lake need to be paid much attention.